Title: Delivery to macrophages and efficacy of glucan particle administered nano-rifabutin
Glucan particles (GP) have lately emerged as 2–4 µm spherical drug delivery systems composed primarily of yeast cell wall derived β-1,3-D-glucan shells. Recently, methods to encapsulate various molecules inside their hollow cavity of these GPs have been developed. β-glucans are natural products, mainly found as cell wall components in fungi, yeast and bacteria and in cereals as barley and oat and have long been considered to improve general health. β-Glucans also act as “natural polysaccharide immunomodulators,” and are known for their ability to activate the immune system. The 1,3-β-glucan outer shell also provides for receptor-mediated uptake by phagocytic cells expressing β-glucan receptors. We report here, the preparation of β-glucan particles (GP) from yeast cells, the preparation of GP loaded with high payload of rifabutin (RB) nano-particles [(RB-NPs)-GP], their characterization and their rapid phagocytic uptake by macrophage. An investigation of the anti-mycobacterial efficacy and cellular activation responses within Mycobacterium tuberculosis (M. tuberculosis) infected J774 macrophage cells following exposure to the (RB-NPs)-GP formulation showed that this formulation enhanced the efficacy of RB drug by approximately 2.5 fold. This data indicates that the [(RB-NPs)-GP] formulation not only targets and activates M. tuberculosis infected macrophage, but also adds significantly to the efficacy of loaded drug molecules.
Dr Rolee Sharma presently working as Associate Professor at the Deptartment Of Biosciences, Integral University, Lucknow, of India. Integral University is a premier educational and research institution recognized from various Goverment Bodies. She received doctoral degree in the year 2006 from Central Drug Research Institute, Lucknow, India. Her research prime objective is to develop biodegradable and biocompatible chemotherapeutic interventions, particularly targeted drug delivery systems against tuberculosis and evaluate the innate immune responses to such formuations. To her credit she has published 42 articles in various international peer reviewed journals.